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新方法实现对单个细胞分裂历史的观测
作者:小柯机器人 发布时间:2021/9/15 13:46:53

近日,瑞士苏黎世大学Sebastian Jessberger团队利用iCOUNT对复杂组织中的单个细胞分裂历史实现可视化分析。2021年9月14日,《细胞—干细胞》杂志在线发表了这项成果。

研究人员开发了一个可诱导的细胞分裂计数器(iCOUNT),并报告了人类和小鼠组织在体外和体内的细胞分裂事件。通过分析发育中和成年大脑中神经干/祖细胞(NSPC)的细胞分裂历史,研究人员表明,iCOUNT能提供对干细胞行为的新见解。

此外,研究人员利用iCOUNT标记的NSPC及其后代的单细胞RNA测序(scRNA-seq),从发育中的小鼠皮层和前脑区域化的人类类器官中鉴定出功能相关的分子途径,这些分子途径在小鼠和人类细胞之间普遍受到调节,并取决于各个细胞的分裂历史。因此,研究人员开发了一个新工具来描述干细胞反复分裂的分子后果,从而能够分析组织形成、平衡和修复的细胞原理。

据悉,单个干细胞的分裂潜力和连续几轮增殖的分子后果在很大程度上仍然是未知的。

附:英文原文

Title: Visualization of individual cell division history in complex tissues using iCOUNT

Author: Annina Denoth-Lippuner, Baptiste N. Jaeger, Tong Liang, Lars N. Royall, Stefanie E. Chie, Kilian Buthey, Diana Machado, Vladislav I. Korobeynyk, Merit Kruse, Clara M. Munz, Alexander Gerbaulet, Benjamin D. Simons, Sebastian Jessberger

Issue&Volume: 2021-09-14

Abstract: The division potential of individual stem cells and the molecular consequences of successive rounds of proliferation remain largely unknown. Here, we developed an inducible cell division counter (iCOUNT) that reports cell division events in human and mouse tissues in vitro and in vivo. Analyzing cell division histories of neural stem/progenitor cells (NSPCs) in the developing and adult brain, we show that iCOUNT can provide novel insights into stem cell behavior. Further, we use single-cell RNA sequencing (scRNA-seq) of iCOUNT-labeled NSPCs and their progenies from the developing mouse cortex and forebrain-regionalized human organoids to identify functionally relevant molecular pathways that are commonly regulated between mouse and human cells, depending on individual cell division histories. Thus, we developed a tool to characterize the molecular consequences of repeated cell divisions of stem cells that allows an analysis of the cellular principles underlying tissue formation, homeostasis, and repair.

DOI: 10.1016/j.stem.2021.08.012

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00349-0

期刊信息

Cell Stem Cell:《细胞—干细胞》,创刊于2007年。隶属于细胞出版社,最新IF:21.464
官方网址:https://www.cell.com/cell-stem-cell/home
投稿链接:https://www.editorialmanager.com/cell-stem-cell/default.aspx